| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
accD
|
1568 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 70.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 15.00% | Poor | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
accD
|
794 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 96.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 22.00% | Low | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
atpF
|
92 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 85.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
clpP
|
559 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 58.00% | Medium | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 36.00% | Low | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
matK
|
706 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 96.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 65.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
1255 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
1481 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
149 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
467 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
586 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 89.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
726 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 16.00% | Poor | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 0.00% | Unedited | Absent | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
746 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
830 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
838 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 77.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhB
|
872 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhD
|
2 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 44.00% | Medium | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 20.00% | Low | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhD
|
383 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhD
|
674 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhD
|
878 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 76.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 65.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhD
|
887 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhF
|
290 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 96.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 50.00% | Medium | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
ndhG
|
50 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 95.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 72.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
petL
|
5 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 58.00% | Medium | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 6.00% | Poor | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
psbE
|
214 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 85.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
psbF
|
77 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 81.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
psbZ
|
50 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 84.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 48.00% | Medium | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpl12
|
NA |
Intron |
NA |
NA |
NA |
58 |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 35.00% | Low | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 38.00% | Low | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpl23
|
89 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 86.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 60.00% | High | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpoA
|
200 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 68.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 47.00% | Medium | Decreased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpoB
|
2432 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 89.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpoB
|
338 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 87.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 89.00% | High | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpoB
|
551 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 90.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 95.00% | High | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rpoC1
|
488 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 52.00% | Medium | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 70.00% | High | Increased | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rps14
|
149 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 75.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 76.00% | High | Similar | 33119889 |
|
| Arabidopsis thaliana |
AT3G06730 |
Chloroplast |
rps14
|
80 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | No treatment | No mutant | Normal | NA | 7 days | Bulk sequencing of RT‐PCR products | 75.00% | High | None | 33119889 | | Salk_028162C | trx z | Knockout | A T‐DNA insertion | Knockout | Albinic | NA | 7 days | Bulk sequencing of RT‐PCR products | 76.00% | High | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
atpA
|
1148 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 95.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 97.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 76.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 97.00% | High | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 85.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 82.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 79.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhA
|
1070 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 88.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 53.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 97.00% | High | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 41.00% | Medium | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 17.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 17.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 44.00% | Medium | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhA
|
473 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 26.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 77.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 11.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 13.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 75.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhA
|
563 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 74.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 78.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 3.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 5.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 69.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
1481 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 94.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 95.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 94.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 92.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 86.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 83.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 91.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
467 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 77.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
586 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 81.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 57.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 42.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 88.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
611 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 85.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 69.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 26.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 17.00% | Poor | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 77.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
704 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
737 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 95.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 96.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 66.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 16.00% | Poor | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 10.00% | Poor | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 8.00% | Poor | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 30.00% | Low | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
830 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 79.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 69.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhB
|
836 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 89.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 82.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhD
|
878 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 89.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 68.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 96.00% | High | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 84.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 47.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 40.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 84.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhF
|
62 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 74.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 89.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 86.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 78.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 84.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 82.00% | High | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhG
|
11 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 75.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 83.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 72.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 49.00% | Medium | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 35.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 35.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 54.00% | Medium | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ndhG
|
347 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 86.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 94.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 66.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 96.00% | High | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 72.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 56.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 52.00% | Medium | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 78.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rpl2
|
2 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 78.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 82.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 71.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 84.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 65.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 68.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 67.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 57.00% | Medium | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rpoB
|
467 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 34.00% | Low | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 60.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 90.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 58.00% | Medium | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 56.00% | Medium | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 83.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 83.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 49.00% | Medium | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rpoB
|
545 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 41.00% | Medium | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 62.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 88.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 60.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 79.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 91.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 91.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 73.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rpoB
|
560 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 40.00% | Medium | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 61.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 86.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 57.00% | Medium | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 78.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 88.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 88.00% | High | Increased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 72.00% | High | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rps14
|
80 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 89.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 31.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 87.00% | High | Restored | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 35.00% | Low | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 22.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 24.00% | Low | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 36.00% | Low | Restored | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
rps8
|
182 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 90.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 94.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 94.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Increased | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 77.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 86.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 85.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 74.00% | High | Similar | 33119889 |
|
| Oryza sativa |
LOC_Os08g29110 |
Chloroplast |
ycf3
|
185 |
CDS |
NA |
NA |
NA |
NA |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Japonica cultivar Nipponbare | WT | Wild Type | Grown at 25℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 25℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | wp2 mutant were indistinguishable from wild‐type seedlings grown at 25°C; Pigment contents and chloroplast ultrastructure in wp2 mutant and wild‐type seedlings were almost identical when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 25℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 25°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 97.00% | High | Similar | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 25℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | NA | NA | 10 days | Bulk sequencing of RT‐PCR products | 100.00% | Complete | Similar | 33119889 | | Japonica cultivar Nipponbare | WT | Wild Type | Grown at 35℃ | No mutant | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 96.00% | High | None | 33119889 | | Japonica cultivar Nipponbare | wp2 | Knockout | Grown at 35℃; A single base transition (G-T) caused substitution of a tryptophan residue (111) by a cysteine residue next to the conserved CXXC motif | Knockout | Seedlings of the wp2 mutant were albinic when grown at 35°C; The pigment contents were significantly decreased at 35°C; Chloroplasts of wp2 were abnormal in shape and contained no thylakoid membranes at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 73.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare | L1 | Knockout | Grown at 35℃; An "A" insertion | Knockout | Seedlings of the L1 mutant were albinic when grown at 35°C | NA | 10 days | Bulk sequencing of RT‐PCR products | 69.00% | High | Decreased | 33119889 | | Japonica cultivar Nipponbare and IR36 (indica) | cp | Complementation | Grown at 35℃; For complementation of the wp2 mutant, the LOC_Os08g29110 coding sequence was cloned into the pCAMBIA1300‐221‐3*FLAG binary vector. The construct was introduced into Agrobacterium tumefa | Knockout | Normal | NA | 10 days | Bulk sequencing of RT‐PCR products | 95.00% | High | Restored | 33119889 |
|
